APPLICATION NOTE

Comparison between scale-X™ bioreactors and microcarrier cultivations

In viral vaccines production, anchorage-dependent cell lines, such as Vero cells are commonly used. Most viral vaccine manufacturing processes are therefore based on surface-dependent cell platforms. Typical cultivation systems such as roller bottles, multi-tray cell culture dishes and microcarrier bioreactors have constraints for large-scale production.

To overcome potential scale-up limitations and to ease manufacturing, fixed-bed bioreactors are a valuable alternative.

In comparison to randomly packed fabric strips found in commonly used fixed-bed bioreactors, the proprietary scale-X™ technology uses a structured, spiral wound fixed-bed to reduce local over compaction and dead zones, while making optimal usage of available surface area with high batch-to-batch reproducibility.

scale-X single-used fixed-bed bioreactor for viral culture - enhanced upstream production - Univercells biotech Belgium

 

Proof of concept for scalable and homogeneous Vero cell growth scale-x fixed-bed bioreactors

Delivering homogeneous cell growth and scalable adherent cell culture

A homogenous cell growth is an important scaling factor. It allows maximum use of an available surface area in a reproducible manner. Ensuring an optimized cell culture can directly influence the yield and productivity of viral particles for vaccine manufacturing.

A study was carried out to compare adherent Vero cell culture in a scale-X hydro bioreactor (2.4 m²) with standard microcarrier cultivations. Univercells has extended this study with complementary cell growth profiles among the scale-X bioreactor range (from 2.4 m² to 30 m²). The valuable obtained results demonstrate the reproducibility and homogeneity of cell growth within scale-X bioreactors, making it a suitable alternative to traditional “scale-out” technologies.

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  • Application note
    | Scale-X

    Univercells scale-X™ bioreactor Vero cell growth

    Proof of concept for scalable and homogeneous Vero cell growth in scale-X hydro (2.4 m²) and carbo (10 m² and 30 m²)